Embryonic zebrafish response to a commercial formulation of azoxystrobin at environmental concentrations
نویسندگان
چکیده
Azoxystrobin is a broad-spectrum strobilurin fungicide for use on wide range of crops available to end-users as formulated products. Due its extensive application, it has been detected in aquatic ecosystems, raising concerns about environmental impact, which still poorly explored. The objective this work was study the effects commercial formulation azoxystrobin zebrafish embryo model. Sublethal and lethal were monitored during exposure period from 2 h post fertilisation (hpf) 96 hpf after concentrations (1, 10 100 μg L−1). responses antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reductase (GR)) well detoxifying (glutathione-s-transferase (GST) carboxylesterase (CarE)) evaluated at hpf. Similarly, levels (reduced (GSH) oxidised (GSSG) glutathione), neurotransmission (acetylcholinesterase (AChE)) anaerobic respiration (lactate dehydrogenase (LDH)) -related assayed. At 120 hpf, larvae each group used behaviour analysis. Results showed concentration-dependent teratogenic effects, particularly by increasing number malformations (yolk eye), with higher prevalence highest concentration. However, found that lowest concentration induced high generation reactive oxygen species (ROS) increased activity SOD, GST, CarE. In addition, GR GSSG decreased concentration, suggesting an adaptive response oxidative stress, also supported AChE absence behavioural changes. These findings advance knowledge developmental impacts, may impose ecotoxicological risks non-target species.
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ژورنال
عنوان ژورنال: Ecotoxicology and Environmental Safety
سال: 2021
ISSN: ['0147-6513', '1090-2414']
DOI: https://doi.org/10.1016/j.ecoenv.2021.111920